Barcelona said on Wednesday that Iniesta, recently named UEFA's Best Player in Europe, pulled a muscle in his right leg during Spain's 1-0 win in Georgia on Tuesday.
Iniesta will miss Saturday's Spanish league game at Getafe as well.
Barcelona also said defender Jordi Alba has the flu and will not return to training until Friday.
Barcelona leads the Spanish league after a perfect start through three rounds, enjoying a five-point advantage over defending champion Real Madrid.
